NASC Stock Detail Page
NASC ID: N849781
Name: WiscDsLox253H09
ABRC stock number: CS849781
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox253H09.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G56160.1 (chr 1 pos 21022787 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796740. Wiscseq_DsLox253H09.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G46320.1 (chr 2 pos 19017276 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796741. Wiscseq_DsLox253H09.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G08000.1 (chr 4 pos 4833164 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796742. Wiscseq_DsLox253H09.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G08092.1 (at chr 4 pos 4986333 (C/4986333-4986420) on the TAIR10). The sequences' 1-88 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 7, C 5 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452397.
Donation Date: 2004-12-01
| Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox253H09
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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